Astaxanthin Treatment Reduced Oxidative Induced Pro-Inflammatory Cytokines Secretion in U937: SHP-1 as a Novel Biological TargetReport as inadecuate




Astaxanthin Treatment Reduced Oxidative Induced Pro-Inflammatory Cytokines Secretion in U937: SHP-1 as a Novel Biological Target - Download this document for free, or read online. Document in PDF available to download.

Department of Medicine and Science of Aging, University G. D’Annunzio-Chieti, Chieti 66100, Italy



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract It has been suggested that oxidative stress activates various intracellular signaling pathways leading to secretion of a variety of pro-inflammatory cytokines and chemokines. SHP-1 is a protein tyrosine phosphatase PTP which acts as a negative regulator of immune cytokine signaling. However, intracellular hydrogen peroxide H2O2, generated endogenously upon stimulation and exogenously from environmental oxidants, has been known to be involved in the process of intracellular signaling through inhibiting various PTPs, including SHP-1. In this study, we investigated the potential role of astaxanthin, an antioxidant marine carotenoid, in re-establishing SHP-1 negative regulation on pro-inflammatory cytokines secretion in U-937 cell line stimulated with oxidative stimulus. ELISA measurement suggested that ASTA treatment 10 µM reduced pro-inflammatory cytokines secretion IL-1β, IL-6 and TNF-α induced through H2O2, 100 µM. Furthermore, this property is elicited by restoration of basal SHP-1 protein expression level and reduced NF-κB p65 nuclear expression, as showed by western blotting experiments. View Full-Text

Keywords: carotenoids; SHP-1 protein; inflammation; astaxanthin carotenoids; SHP-1 protein; inflammation; astaxanthin





Author: Lorenza Speranza †,,* , Mirko Pesce †, Antonia Patruno, Sara Franceschelli, Maria Anna de Lutiis, Alfredo Grilli and Mario Felaco

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents