Structural Characterization and Anti-HSV-1 and HSV-2 Activity of Glycolipids from the Marine Algae Osmundaria obtusiloba Isolated from Southeastern Brazilian CoastReport as inadecuate




Structural Characterization and Anti-HSV-1 and HSV-2 Activity of Glycolipids from the Marine Algae Osmundaria obtusiloba Isolated from Southeastern Brazilian Coast - Download this document for free, or read online. Document in PDF available to download.

1

Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba 81531-990, PR, Brazil

2

Department of Virology , Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil

3

Department of General Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil





*

Author to whom correspondence should be addressed.



Abstract Glycolipids were extracted from the red alga Osmundaria obtusiloba from Southeastern Brazilian coast. The acetone insoluble material was extracted with chloroform-methanol and the lipids, enriched in glycolipids, were fractionated on a silica gel column eluted with chloroform, acetone and then methanol. Three major orcinol-positive bands were found in the acetone and methanol fractions, being detected by thin layer chromatography. The structures of the corresponding glycolipids were elucidated by ESI-MS and 1H-13C NMR analysis, on the basis of their tandem-MS behavior and HSQC, TOCSY fingerprints. For the first time, the structure of sulfoquinovosyldiacylglycerol from the red alga Osmundaria obtusiloba was characterized. This molecule exhibited potent antiviral activity against HSV-1 and HSV-2 with EC50 values of 42 µg-mL to HSV-1 and 12 µg-mL to HSV-2, respectively. Two other glycolipids, mono- and digalactosyldiacylglycerol, were also found in the alga, being characterized by ESI-MS-MS. The structural elucidation of algae glycolipids is a first step for a better understanding of the relation between these structures and their biological activities. View Full-Text

Keywords: Osmundaria obtusiloba; red alga; glycolipids; sulfoquinovosyldiacylglycerol; anti-herpes simplex activity Osmundaria obtusiloba; red alga; glycolipids; sulfoquinovosyldiacylglycerol; anti-herpes simplex activity





Author: Lauro M. de Souza 1, Guilherme L. Sassaki 1, Maria Teresa Villela Romanos 2 and Eliana Barreto-Bergter 3,*

Source: http://mdpi.com/



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