Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish Saurida elongata Muscle Protein HydrolysateReport as inadecuate




Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish Saurida elongata Muscle Protein Hydrolysate - Download this document for free, or read online. Document in PDF available to download.

1

School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China

2

Department of Pharmacy, Liuzhou Medical College, Liuzhou, Guangxi 545006, China

3

Guangxi Key Laboratory of Petrochemical Resources Processing & Process Intensification Technology, Nanning, Guangxi 530004, China





*

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Abstract Lizard fish Saurida elongata muscle protein was hydrolyzed using neutral protease to produce protein hydrolysate LFPH, and the hydrolysis conditions were investigated using response-surface methodology. The optimum conditions for producing peptides with the highest angiotensin-I converting enzyme ACE-inhibitory activity were the following: enzyme-to-substrate ratio of 10,000 U-g, temperature of 48 °C, pH 7.0, and hydrolysis time of 2 h. Under these conditions, the ACE-inhibitory activity of LFPH and the degree of hydrolysis were 84% and 24%, respectively. A novel ACE-inhibitory peptide was isolated from LFPH using ultrafiltration, Sephadex G-15, and high-performance liquid chromatography. The amino acid sequence of the ACE-inhibitory peptide was identified as Ser-Pro-Arg-Cys-Arg SPRCR, and its IC50 was 41 ± 1 µM. View Full-Text

Keywords: ACE-inhibitory peptide; lizard fish; enzymatic hydrolysis; response surface methodology; isolation ACE-inhibitory peptide; lizard fish; enzymatic hydrolysis; response surface methodology; isolation





Author: Shanguang Wu 1,2, Jianhua Sun 1,3, Zhangfa Tong 1,3, Xiongdiao Lan 1, Zhongxing Zhao 1 and Dankui Liao 1,3,*

Source: http://mdpi.com/



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