The Antiangiogenic Compound Aeroplysinin-1 Induces Apoptosis in Endothelial Cells by Activating the Mitochondrial PathwayReport as inadecuate




The Antiangiogenic Compound Aeroplysinin-1 Induces Apoptosis in Endothelial Cells by Activating the Mitochondrial Pathway - Download this document for free, or read online. Document in PDF available to download.

1

Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, Málaga E-29071, Spain

2

Genes and Cancer Group, Cancer Epigenetics and Biology Program PEBC-IDIBELL, Barcelona E-08908, Spain

3

Centre for Biomedical Network Research on Rare Diseases CIBERER, Málaga E-29071, Spain





*

Author to whom correspondence should be addressed.



Abstract Aeroplysinin-1 is a brominated metabolite extracted from the marine sponge Aplysina aerophoba that has been previously characterized by our group as a potent antiangiogenic compound in vitro and in vivo. In this work, we provide evidence of a selective induction of apoptosis by aeroplysinin-1 in endothelial cells. Studies on the nuclear morphology of treated cells revealed that aeroplysinin-1 induces chromatin condensation and nuclear fragmentation, and it increases the percentage of cells with sub-diploid DNA content in endothelial, but not in HCT-116, human colon carcinoma and HT-1080 human fibrosarcoma cells. Treatment of endothelial cells with aeroplysinin-1 induces activation of caspases-2 -3 -8 and -9, as well as the cleavage of apoptotic substrates, such as poly ADP-ribose polymerase and lamin-A in a caspase-dependent mechanism. Our data indicate a relevant role of the mitochondria in the apoptogenic activity of this compound. The observation that aeroplysinin-1 prevents the phosphorylation of Bad relates to the mitochondria-mediated induction of apoptosis by this compound. View Full-Text

Keywords: aeroplysinin-1; angiogenesis; apoptosis; caspases aeroplysinin-1; angiogenesis; apoptosis; caspases





Author: Beatriz Martínez-Poveda 1, Salvador Rodríguez-Nieto 2, Melissa García-Caballero 1, Miguel-Ángel Medina 1,3 and Ana R. Quesada 1,3,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents