Bioactive Potential of Marine Macroalgae from the Central Red Sea Saudi Arabia Assessed by High-Throughput Imaging-Based Phenotypic ProfilingReport as inadecuate


Bioactive Potential of Marine Macroalgae from the Central Red Sea Saudi Arabia Assessed by High-Throughput Imaging-Based Phenotypic Profiling


Bioactive Potential of Marine Macroalgae from the Central Red Sea Saudi Arabia Assessed by High-Throughput Imaging-Based Phenotypic Profiling - Download this document for free, or read online. Document in PDF available to download.

1

Red Sea Research Center, Division of Biological and Environmental Science and Engineering BESE, King Abdullah University of Science and Technology KAUST, 23955-6900 Thuwal, Saudi Arabia

2

Research Unit Analytical Biogeochemistry, Helmholtz Zentrum München, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany

3

Chair of Analytical Food Chemistry, Technische Universität München TUM, 85354 Freising-Weihenstephan, Germany





*

Author to whom correspondence should be addressed.



Academic Editor: Sylvia Urban

Abstract Marine algae represent an important source of novel natural products. While their bioactive potential has been studied to some extent, limited information is available on marine algae from the Red Sea. This study aimed at the broad discovery of new bioactivities from a collection of twelve macroalgal species from the Central Red Sea. We used imaging-based High-Content Screening HCS with a diverse spectrum of cellular markers for detailed cytological profiling of fractionated algal extracts. The cytological profiles for 3 out of 60 algal fractions clustered closely to reference inhibitors and showed strong inhibitory activities on the HIV-1 reverse transcriptase in a single-enzyme biochemical assay, validating the suggested biological target. Subsequent chemical profiling of the active fractions of two brown algal species by ultra-high resolution mass spectrometry FT-ICR-MS revealed possible candidate molecules. A database query of these molecules led us to groups of compounds with structural similarities, which are suggested to be responsible for the observed activity. Our work demonstrates the versatility and power of cytological profiling for the bioprospecting of unknown biological resources and highlights Red Sea algae as a source of bioactives that may serve as a starting point for further studies. View Full-Text

Keywords: bioprospecting; natural products; algae; cytological profiling; High-Content Screening; HIV bioprospecting; natural products; algae; cytological profiling; High-Content Screening; HIV





Author: Stephan Kremb 1, Constanze Müller 2, Philippe Schmitt-Kopplin 2,3 and Christian R. Voolstra 1,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents