The Influence of Tetrodotoxin TTX on the Distribution and Chemical Coding of Caudal Mesenteric Ganglion CaMG Neurons Supplying the Porcine Urinary BladderReport as inadecuate


The Influence of Tetrodotoxin TTX on the Distribution and Chemical Coding of Caudal Mesenteric Ganglion CaMG Neurons Supplying the Porcine Urinary Bladder


The Influence of Tetrodotoxin TTX on the Distribution and Chemical Coding of Caudal Mesenteric Ganglion CaMG Neurons Supplying the Porcine Urinary Bladder - Download this document for free, or read online. Document in PDF available to download.

1

Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, Poland

2

Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

3

Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn,10-719 Olsztyn, Poland





*

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Academic Editor: Peer B. Jacobson

Abstract The treatment of micturition disorders creates a serious problem for urologists. Recently, new therapeutic agents, such as neurotoxins, are being considered for the therapy of urological patients. The present study investigated the chemical coding of caudal mesenteric ganglion CaMG neurons supplying the porcine urinary bladder after intravesical instillation of tetrodotoxin TTX. The CaMG neurons were visualized with retrograde tracer Fast blue FB and their chemical profile was disclosed with double-labeling immunohistochemistry using antibodies against tyrosine hydroxylase TH, neuropeptide Y NPY, vasoactive intestinal polypeptide VIP, somatostatin SOM, calbindin CB, galanin GAL and neuronal nitric oxide synthase nNOS. It was found that in both the control n = 6 and TTX-treated pigs n = 6, the vast majority 92.6% ± 3.4% and 88.8% ± 2%, respectively of FB-positive FB+ nerve cells were TH+. TTX instillation caused a decrease in the number of FB+-TH+ neurons immunopositive to NPY 88.9% ± 5.3% in the control animals vs. 10.6% ± 5.3% in TTX-treated pigs or VIP 1.7% ± 0.6% vs. 0%, and an increase in the number of FB+-TH+ neurons immunoreactive to SOM 8.8% ± 1.6% vs. 39% ± 12.8%, CB 1.8% ± 0.7% vs. 12.6% ± 2.7%, GAL 1.7% ± 0.8% vs. 10.9% ± 2.6% or nNOS 0% vs. 1.1% ± 0.3%. The present study is the first to suggest that TTX modifies the chemical coding of CaMG neurons supplying the porcine urinary bladder. View Full-Text

Keywords: tetrodotoxin; caudal mesenteric ganglion; urinary bladder; immunohistochemistry; pig tetrodotoxin; caudal mesenteric ganglion; urinary bladder; immunohistochemistry; pig





Author: Ewa Lepiarczyk 1,* , Agnieszka Bossowska 1, Jerzy Kaleczyc 2, Marta Majewska 1, Sławomir Gonkowski 3 and Mariusz Majewski 1

Source: http://mdpi.com/



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