Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 PreadipocytesReport as inadecuate


Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 Preadipocytes


Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 Preadipocytes - Download this document for free, or read online. Document in PDF available to download.

1

Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea

2

Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02445, USA

3

Department of Animal Bio and Applied Chemistry, Konkuk University, Chungju 27478, Korea

4

Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science &Technology KIOST, Jeju 63349, Korea

5

Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas-UNICAMP, Campinas 02134, Brazil

6

Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju 27478, Korea



These authors contributed equally to this study.





*

Authors to whom correspondence should be addressed.



Academic Editors: Allen Place, Paul Long, Rosemary Jagus and Joy Watts

Abstract Seaweed, a popular and abundant food ingredient mainly consumed in Asian countries, is a good source of bioactive compounds with anti-obesity effects. However, the anti-obesity effects of Sargassum thunbergii have not yet been established. In this study, we isolated six indole derivatives STCs—indole-2-carboxaldehyde STC-1, indole-3-carboxaldehyde STC-2, indole-4-carboxaldehyde STC-3, indole-5-carboxaldehyde STC-4, indole-6-carboxaldehyde STC-5, and indole-7-carboxaldehyde STC-6—from S. thunbergii and evaluated their inhibitory effects on adipocyte differentiation in 3T3-L1 cells. We found that STC-1 and STC-5 resulted in non-toxic inhibition of the differentiation of 3T3-L1 adipocytes and thus selected these compounds for further study. STC-1 and STC-5 significantly inhibited lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor-γ PPARγ, CCAAT-enhancer-binding protein α C-EBPα, and sterol regulatory element-binding protein 1c SREBP-1c in a dose-dependent manner. The specific mechanism mediating the effects of STC-1 and STC-5 was shown to be AMP-activated protein kinase AMPK activation. Our results demonstrated the inhibitory effect of STC-1 and STC-5 on adipogenesis through the activation of the AMPK signal pathway. Together, these findings suggested that STC-1 and STC-5 may be effective candidates for the prevention of obesity or obesity-related diseases. View Full-Text

Keywords: anti-obesity effect; adipocyte; Sargassum thunbergii; indole derivatives anti-obesity effect; adipocyte; Sargassum thunbergii; indole derivatives





Author: Min-Cheol Kang 1,2,†, Yuling Ding 3,†, Eun-A Kim 4, Youn Kyung Choi 4, Thiago de Araujo 5, Soo-Jin Heo 4,* and Seung-Hong Lee 6,*

Source: http://mdpi.com/



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