Anti-Pigmentary Effect of -4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.Report as inadecuate


Anti-Pigmentary Effect of -4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.


Anti-Pigmentary Effect of -4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp. - Download this document for free, or read online. Document in PDF available to download.

1

College of Pharmacy, Ewha Womans University, Seoul 03760, Korea

2

Department of Chemistry and Nano Science, Global Top 5 Program, Ewha Womans University, Seoul 03760, Korea

3

Department of Biology, College of Life Science and Natural Resource, Sunchon National University, Suncheon 57922, Korea





*

Authors to whom correspondence should be addressed.



Abstract Bioactivity-guided isolation of a crude extract from a culture broth of Bacillus sp. has led to the isolation of -4-hydroxysattabacin 1. The inhibitory effect of -4-hydroxysattabacin 1 was investigated on melanogenesis in the murine melanoma cell line, B16F10, and human melanoma cell line, MNT-1, as well as a pigmented 3D-human skin model. -4-Hydroxysattabacin treatment decreased melanin contents in a dose-dependent manner in α-melanocyte stimulating hormone α-MSH-stimulated B16F10 cells. Quantitative real time PCR qRT–PCR demonstrated that treatment with -4-hydroxysattabacin down-regulated several melanogenic genes, including tyrosinase, tyrosinase-related protein 1 TRP-1, and tyrosinase-related protein 2 TRP-2 while their enzymatic activities were unaffected. The anti-melanogenic effects of -4-hydroxysattabacin were further demonstrated in a pigmented 3D human epidermal skin model, MelanodermTM, and manifested as whitening and regression of melanocyte activation in the tissue. View Full-Text

Keywords: Bacillus sp.; -4-hydroxysattabacin; melanogenesis; marine-derived; anti-pigmentary effect Bacillus sp.; -4-hydroxysattabacin; melanogenesis; marine-derived; anti-pigmentary effect





Author: Kyuri Kim 1, Alain S. Leutou 2, Haein Jeong 2, Dayoung Kim 2, Chi Nam Seong 3, Sang-Jip Nam 2,* and Kyung-Min Lim 1,*

Source: http://mdpi.com/



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