Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from MarineReport as inadecuate


Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine


Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine - Download this document for free, or read online. Document in PDF available to download.

1

College of Environment, Zhejiang University of Technology, Hangzhou 310014, China

2

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

3

Nanjing Yuanheng Institute for Environmental Studies Co., Ltd., Nanjing 210049, China



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Academic Editors: Vassilios Roussis, Efstathia Ioannou and Peer B. Jacobson

Abstract The presence of nicotine and nicotinic acid NA in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-degrading bacterium—strain JQ581—was isolated from sediment from the East China Sea and identified as a member of Pseudomonas putida based on morphology, physio-biochemical characteristics, and 16S rDNA gene analysis. The relationship between growth and nicotine-NA degradation suggested that strain JQ581 was a good candidate for applications in the bioaugmentation treatment of nicotine-NA contamination. The degradation intermediates of nicotine are pseudooxynicotine PN and 3-succinoyl-pyridine SP based on UV, high performance liquid chromatography, and liquid chromatography-mass spectrometry analyses. However, 6-hydroxy-3-succinoyl-pyridine HSP was not detected. NA degradation intermediates were identified as 6-hydroxynicotinic acid 6HNA. The whole genome of strain JQ581 was sequenced and analyzed. Genome sequence analysis revealed that strain JQ581 contained the gene clusters for nicotine and NA degradation. This is the first report where a marine-derived Pseudomonas strain had the ability to degrade nicotine and NA simultaneously. View Full-Text

Keywords: nicotine; nicotinic acid; biodegradation; genome; Pseudomonas putida; marine-derived bacterium nicotine; nicotinic acid; biodegradation; genome; Pseudomonas putida; marine-derived bacterium





Author: Aiwen Li 1,†, Jiguo Qiu 1,2,†, Dongzhi Chen 1, Jiexu Ye 1, Yuhong Wang 3, Lu Tong 3, Jiandong Jiang 2,* and Jianmeng Chen 1,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents