Estrogen Stimulates Proliferation and Differentiation of Neural Stem-Progenitor Cells through Different Signal Transduction PathwaysReport as inadecuate




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1

Laboratory of Molecular Biology, Department of Biofunctional Analysis, Gifu Pharmaceutical University, Gifu, Japan

2

Department of Pharmaceutical Pharmacology, Faculty of Pharmaceutical Sciences, Matsuyama University, Ehime, Japan





*

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Abstract Our previous study indicated that both 17β-estradiol E2, known to be an endogenous estrogen, and bisphenol A BPA, known to be a xenoestrogen, could positively influence the proliferation or differentiation of neural stem-progenitor cells NS-PCs. The aim of the present study was to identify the signal transduction pathways for estrogenic activities promoting proliferation and differentiation of NS-PCs via well known nuclear estrogen receptors ERs or putative membrane-associated ERs. NS-PCs were cultured from the telencephalon of 15-day-old rat embryos. In order to confirm the involvement of nuclear ERs for estrogenic activities, their specific antagonist, ICI-182,780, was used. The presence of putative membrane-associated ER was functionally examined as to whether E2 can activate rapid intracellular signaling mechanism. In order to confirm the involvement of membrane-associated ERs for estrogenic activities, a cell-impermeable E2, bovine serum albumin-conjugated E2 E2-BSA was used. We showed that E2 could rapidly activate extracellular signal-regulated kinases 1-2 ERK 1-2, which was not inhibited by ICI-182,780. ICI-182,780 abrogated the stimulatory effect of these estrogens E2 and BPA on the proliferation of NS-PCs, but not their effect on the differentiation of the NS-PCs into oligodendroglia. Furthermore, E2-BSA mimicked the activity of differentiation from NS-PCs into oligodendroglia, but not the activity of proliferation. Our study suggests that 1 the estrogen induced proliferation of NS-PCs is mediated via nuclear ERs; 2 the oligodendroglial generation from NS-PCs is likely to be stimulated via putative membrane‑associated ERs. View Full-Text

Keywords: 17β-estradiol; bisphenol A; estrogen; neural stem cell; oligodendrocytes; proliferation; differeciation 17β-estradiol; bisphenol A; estrogen; neural stem cell; oligodendrocytes; proliferation; differeciation





Author: Makiko Okada 1, Akihisa Makino 1, Mitsunari Nakajima 2, Satoshi Okuyama 2, Shoei Furukawa 1 and Yoshiko Furukawa 1,2,*

Source: http://mdpi.com/



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