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1

Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China

2

Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China

3

Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China





*

Authors to whom correspondence should be addressed.



Abstract In this work, we report the studies of drug metabolism by xanthine oxidase XOD with electrochemical techniques. Firstly, a pair of stable, well-defined and quasi-reversible oxidation-reduction peaks is obtained with the formal potential at −413.1 mV vs. SCE after embedding XOD in salmon sperm DNA membrane on the surface of pyrolytic graphite electrode. Then, a new steady peak can be observed at −730 mV vs. SCE upon the addition of 6-mercaptopurine 6-MP to the electrochemical system, indicating the metabolism of 6-MP by XOD. Furthermore, the chronoamperometric response shows that the current of the catalytic peak located at −730 mV increases with addition of 6-MP in a concentration-dependent manner, and the increase of the chronoamperometric current can be inhibited by an XOD inhibitor, quercetin. Therefore, our results prove that XOD-DNA modified electrode can be efficiently used to study the metabolism of 6-MP, which may provide a convenient approach for in vitro studies on enzyme-catalyzed drug metabolism. View Full-Text

Keywords: xanthine oxidase; electrochemistry; drug metabolism; 6-mercaptopurine; quercetin xanthine oxidase; electrochemistry; drug metabolism; 6-mercaptopurine; quercetin





Author: Jing Zhao 1, Xiaolin He 1, Nana Yang 2, Lizhou Sun 2,* and Genxi Li 1,3,*

Source: http://mdpi.com/



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