Development of Eighteen Microsatellite Markers in Anemone amurensis Ranunculaceae and Cross-Amplification in Congeneric SpeciesReport as inadecuate




Development of Eighteen Microsatellite Markers in Anemone amurensis Ranunculaceae and Cross-Amplification in Congeneric Species - Download this document for free, or read online. Document in PDF available to download.

Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China





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Abstract Polyploidy plays an important role in the evolution of plant genomes. To enable the investigation of the polyploidy events within the genus Anemone, we developed eighteen microsatellite markers from the hexaploid species A. amurensis Ranunculaceae, and tested their transferability in five closely related species. The number of total alleles NA for each resulting locus varied from one to eight. The polymorphism information content PIC and Nei’s genetic diversity NGD for these microsatellites ranged from 0.00 to 0.71 and 0.00 to 0.91, respectively. For each population, the NA was one to seven, and the values of PIC and NGD varied from 0.00 to 0.84 and 0.00 to 0.95, respectively. In addition, most of these microsatellites can be amplified successfully in the congeneric species. These microsatellite primers provide us an opportunity to study the polyploid evolution in the genus Anemone. View Full-Text

Keywords: Anemone; A. amurensis; microsatellite; polyploidy; polymorphism information content Anemone; A. amurensis; microsatellite; polyploidy; polymorphism information content





Author: Mingzhou Sun, Xiao Yin, Fengxue Shi, Lin Li, Mingrui Li, Linfeng Li and Hongxing Xiao *

Source: http://mdpi.com/



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