Genome-Wide Identification and in Silico Analysis of Poplar Peptide DeformylasesReport as inadecuate




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1

State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China

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Laboratory for Chemical Defense and Microscale Analysis, P.O. Box 3, Zhijiang 443200, China

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Forestry College, Beihua University, Jilin 132013, China

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School of Basic Medical Sciences, Jiamusi University, Jiamusi 154000, China

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Forestry Research Institution of Heilongjiang Province, Harbin 150081, China





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Abstract Peptide deformylases PDF behave as monomeric metal cation hydrolases for the removal of the N-formyl group Fo. This is an essential step in the N-terminal Met excision NME that occurs in these proteins from eukaryotic mitochondria or chloroplasts. Although PDFs have been identified and their structure and function have been characterized in several herbaceous species, it remains as yet unexplored in poplar. Here, we report on the first identification of two genes PtrPDF1A and PtrPDF1B respectively encoding two putative PDF polypeptides in Populus trichocarpa by genome-wide investigation. One of them XP 002300047.1 encoded by PtrPDF1B XM 002300011.1 was truncated, and then revised into a complete sequence based on its ESTs support with high confidence. We document that the two PDF1s of Populus are evolutionarily divergent, likely as a result of independent duplicated events. Furthermore, in silico simulations demonstrated that PtrPDF1A and PtrPDF1B should act as similar PDF catalytic activities to their corresponding PDF orthologs in Arabidopsis. This result would be value of for further assessment of their biological activities in poplar, and further experiments are now required to confirm them. View Full-Text

Keywords: peptide deformylase; N-terminal Met excision; in silico simulation; genome-wide investigation; phylogenetic analysis; gene duplication; ghromosome location; gene structure display peptide deformylase; N-terminal Met excision; in silico simulation; genome-wide investigation; phylogenetic analysis; gene duplication; ghromosome location; gene structure display





Author: Chang-Cai Liu 1,2, Bao-Guang Liu 3, Zhi-Wei Yang 4, Chun-Ming Li 5, Bai-Chen Wang 1 and Chuan-Ping Yang 1,*

Source: http://mdpi.com/



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