Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode SymbiontReport as inadecuate




Purification and Properties of an Insecticidal Metalloprotease Produced by Photorhabdus luminescens Strain 0805-P5G, the Entomopathogenic Nematode Symbiont - Download this document for free, or read online. Document in PDF available to download.

1

Institute of Biotechnology and Bioinformatics, Asia University, Wufeng, Taichung 413, Taiwan

2

Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung 824, Taiwan

3

Biopesticides Division, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Wufeng, Taichung 413, Taiwan

4

Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan





*

Authors to whom correspondence should be addressed.



Abstract A total of 13 Photorhabdus luminescens strains were screened for proteolytic activity. The P. luminescens strain 0805-P5G had the highest activity on both skim milk and gelatin plates. The protease was purified to electrophoretical homogeneity by using a two-step column chromatographic procedure. It had a molecular weight of 51.8 kDa, as determined by MALDI-TOF mass spectrometry. The optimum pH, temperature, as well as pH and thermal stabilities were 8, 60 °C, 5–10, and 14–60 °C, respectively. It was completely inhibited by EDTA and 1,10-phenanthroline. Bioassay of the purified protease against Galleria mellonella by injection showed high insecticidal activity. The protease also showed high oral toxicity to the diamondback moth Plutella xylostella of a Taiwan field-collected strain, but low toxicity to an American strain. To our knowledge, this is the first report to demonstrate that the purified protease of P. luminescens has direct toxicity to P. xylostella and biopesticide potentiality. View Full-Text

Keywords: protease; Photorhabdus luminescens; Plutella xylostella; insecticidal activity protease; Photorhabdus luminescens; Plutella xylostella; insecticidal activity





Author: Yu-Tzu Chang 1, Chienyan Hsieh 2, Li-Ching Wu 3, Hebron C. Chang 1, Suey-Sheng Kao 3, Menghsiao Meng 4,* and Feng-Chia Hsieh 3,*

Source: http://mdpi.com/



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