Proteome and Peptidome of Human Acquired Enamel Pellicle on Deciduous TeethReport as inadecuate




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Schulich School of Medicine & Dentistry, The University Western University, London, ON N5A6C1, Canada





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Abstract Understanding the composition and structure of the acquired enamel pellicle AEP has been a major goal in oral biology. Our lab has conducted studies on the composition of AEP formed on permanent enamel. The exhaustive exploration has provided a comprehensive identification of more than 100 proteins from AEP formed on permanent enamel. The AEP formed on deciduous enamel has not been subjected to the same biochemical characterization scrutiny as that of permanent enamel, despite the fact that deciduous enamel is structurally different from permanent enamel. We hypothesized that the AEP proteome and peptidome formed on deciduous enamel may also be composed of unique proteins, some of which may not be common with AEP of permanent enamel explored previously. Pellicle material was collected from 10 children aged 18–54 months and subjected to mass spectrometry analysis. A total of 76 pellicle proteins were identified from the deciduous pellicle proteome. In addition, 38 natural occurring AEP peptides were identified from 10 proteins, suggesting that primary AEP proteome-peptidome presents a unique proteome composition. This is the first study to provide a comprehensive investigation of in vivo AEP formed on deciduous enamel. View Full-Text

Keywords: acquired enamel pellicle; saliva; proteomics; primary teeth; proteins; oral; LC-MS-MS acquired enamel pellicle; saliva; proteomics; primary teeth; proteins; oral; LC-MS-MS





Author: Jason N. Zimmerman, William Custodio, Sahza Hatibovic-Kofman, Young Ho Lee, Yizhi Xiao and Walter L. Siqueira *

Source: http://mdpi.com/



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