Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in MiceReport as inadecuate




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1

Departments of Anatomy, Weifang Medical University, Weifang 261053, China

2

Departments of Pathology, Weifang Medical University, Weifang 261053, China

3

Department of Neurosurgery, Brigham and Womens Hospital, Harvard Medical School, Boston, MA 02115, USA



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Abstract The purpose of this study was to investigate the possible protective effect of N-acetylserotonin NAS against acute hepatic ischemia-reperfusion I-R injury in mice. Adult male mice were randomly divided into three groups: sham, I-R, and I-R + NAS. The hepatic I-R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin HE and terminal deoxynucleotidyl transferase dUTP nick end labeling TUNEL staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase AST, malondialdehyde MDA, and superoxide dismutase SOD was evaluated by enzyme-linked immunosorbent assay ELISA. The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury. View Full-Text

Keywords: N-acetylserotonin; newborn mouse; hepatic ischemia-reperfusion injury; apoptosis N-acetylserotonin; newborn mouse; hepatic ischemia-reperfusion injury; apoptosis





Author: Shuna Yu 1,†, Jie Zheng 2,†, Zhengchen Jiang 1, Caixing Shi 1, Jin Li 1, Xiaodong Du 1, Hailiang Wang 1, Jiying Jiang 1,* and Xin Wang 3,*

Source: http://mdpi.com/



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