Increased Chondrocyte Apoptosis Is Associated with Progression of Osteoarthritis in Spontaneous Guinea Pig Models of the DiseaseReport as inadecuate




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1

Centre for Comparative and Clinical Anatomy, University of Bristol, Southwell Street, Bristol BS2 8EJ, UK

2

School of Veterinary Science, University of Bristol, Bristol BS8 1TH, UK





*

Author to whom correspondence should be addressed.



Abstract Osteoarthritis OA is the most common joint disease characterised by degradation of articular cartilage and bone remodelling. For almost a decade chondrocyte apoptosis has been investigated as a possible mechanism of cartilage damage in OA, but its precise role in initiation and-or progression of OA remains to the determined. The aim of this study is to determine the role of chondrocyte apoptosis in spontaneous animal models of OA. Right tibias from six male Dunkin Hartley DH and Bristol Strain 2 BS2 guinea pigs were collected at 10, 16, 24 and 30 weeks of age. Fresh-frozen sections of tibial epiphysis were microscopically scored for OA, and immunostained with caspase-3 and TUNEL for apoptotic chondrocytes. The DH strain had more pronounced cartilage damage than BS2, especially at 30 weeks. At this time point, the apoptotic chondrocytes were largely confined to the deep zone of articular cartilage AC with a greater percentage in the medial side of DH than BS2 DH: 5.7%, 95% CI: 4.2–7.2, BS2: 4.8%, 95% CI: 3.8–5.8, p > 0.05. DH had a significant progression of chondrocyte death between 24 to 30 weeks during which time significant changes were observed in AC fibrillation, proteoglycan depletion and overall microscopic OA score. A strong correlation p ≤ 0.01 was found between chondrocyte apoptosis and AC fibrillation r = 0.3, cellularity r = 0.4 and overall microscopic OA scores r = 0.4. Overall, the rate of progression in OA and apoptosis over the study period was greater in the DH versus BS2 and the medial AC versus lateral. Chondrocyte apoptosis was higher at the later stage of OA development when the cartilage matrix was hypocellular and highly fibrillated, suggesting that chondrocyte apoptosis is a late event in OA. View Full-Text

Keywords: chondrocyte apoptosis; osteoarthritis; animal models; disease progression; caspase-3 chondrocyte apoptosis; osteoarthritis; animal models; disease progression; caspase-3





Author: Zaitunnatakhin Zamli 1, Michael A. Adams 1, John F. Tarlton 2 and Mohammed Sharif 1,*

Source: http://mdpi.com/



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