Celf1 Is Required for Formation of Endoderm-Derived Organs in ZebrafishReport as inadecuate




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Gene Regulation Research, Nara Institute Science and Technology, 8916-5 Takayama, Nara 630-0101, Japan





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Abstract We recently reported that an RNA binding protein called Cugbp Elav-like family member 1 Celf1 regulates somite symmetry and left-right patterning in zebrafish. In this report, we show additional roles of Celf1 in zebrafish organogenesis. When celf1 is knocked down by using an antisense morpholino oligonucleotides MO, liver buds fail to form, and pancreas buds do not form a cluster, suggesting earlier defects in endoderm organogenesis. As expected, we found failures in endoderm cell growth and migration during gastrulation in embryos injected with celf1-MOs. RNA immunoprecipitation revealed that Celf1 binds to gata5 and cdc42 mRNAs which are known to be involved in cell growth and migration, respectively. Our results therefore suggest that Celf1 regulates proper organogenesis of endoderm-derived tissues by regulating the expression of such targets. View Full-Text

Keywords: post-transcriptional regulation; endoderm; liver; pancreas; movement; proliferation post-transcriptional regulation; endoderm; liver; pancreas; movement; proliferation





Author: Naoyuki Tahara, Yasumasa Bessho and Takaaki Matsui *

Source: http://mdpi.com/



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