Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with OsteosarcomasReport as inadecuate




Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas - Download this document for free, or read online. Document in PDF available to download.

1

Department of Orthopedic Surgery, the Second Hospital Affiliated to Xian Jiao Tong University School of Medicine, Xian 710004, Shaanxi, China

2

Department of Aesthetic Plastic Surgery, the Second Hospital Affiliated to Xian Jiao Tong University School of Medicine, Xian 710004, Shaanxi, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract Aim: To investigate whether the aberrant expression of microRNA miR-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma. Methods: Quantitative real-time reverse transcriptase-polymerase chain reaction qRT-PCR analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients’ sera. Results: Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues both p < 0.001, in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls both p < 0.001. Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade p = 0.008 and 0.01, respectively, positive metastasis p = 0.001 and 0.006, respectively and recurrence p = 0.001 and 0.006, respectively. Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a-miR-196b were all independent prognostic factors for OS overall survival and DFS disease-free survival of osteosarcoma patients. Conclusion: Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy. View Full-Text

Keywords: osteosarcoma; microRNA-196a; microRNA-196b; serum; clinicopathological features; overall survival; disease-free survival osteosarcoma; microRNA-196a; microRNA-196b; serum; clinicopathological features; overall survival; disease-free survival





Author: Chun Zhang 1,†, Cong Yao 2,†, Haopeng Li 1, Guoyu Wang 1 and Xijng He 1,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents