Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine TransferaseReport as inadecuate




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1

Department Microbiology and Parasitology, Faculty Pharmacy, University of Barcelona, Av. Joan XXIII s-n, 08028 Barcelona, Spain

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Department Microbiology, Faculty Biology, University Barcelona, Diagonal 643, 08071 Barcelona, Spain

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N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia



In memoriam.





*

Author to whom correspondence should be addressed.



Abstract By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide LPS phosphoethanolamine transferases, three putative candidates PMI3040, PMI3576, and PMI3104 were identified. One of them, eptC PMI3104 was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC PMI3104 product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains. View Full-Text

Keywords: Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine





Author: Eleonora Aquilini 1,2, Susana Merino 2, Yuriy A. Knirel 3, Miguel Regué 1,† and Juan M. Tomás 2,*

Source: http://mdpi.com/



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