A New Type I Peritrophic Membrane Protein from Larval Holotrichia oblita Coleoptera: Melolonthidae Binds to ChitinReport as inadecuate




A New Type I Peritrophic Membrane Protein from Larval Holotrichia oblita Coleoptera: Melolonthidae Binds to Chitin - Download this document for free, or read online. Document in PDF available to download.

1

Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei, China

2

Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China

3

College of Plant Protection, Agricultural University of Hebei-Biological Control Centre of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, Hebei, China

4

Shijiazhuang Development and Reform Commission, Shijiazhuang 050011, Hebei, China





*

Author to whom correspondence should be addressed.



Abstract Peritrophic membranes PMs are composed of chitin and protein. Chitin and protein play important roles in the structural formation and function of the PM. A new type I PM protein, HoCBP76, was identified from the Holotrichia oblita. HoCBP76 was shown as a 62.3 kDa protein by SDS-PAGE analysis and appeard to be associated with the PM throughout its entire length. In H. oblita larvae, the midgut is the only tissue where HoCBP76 could be detected during the feeding period of the larvae. The predicted amino acid sequence indicates that it contains seven tandem chitin binding domains belonging to the peritrophin-A family. HoCBP76 has chitin binding activity and is strongly associated with the PM. The HoCBP76 was not a mucin-like glycoprotein, and the consensus of conserved cysteines appeared to be CX13–17CX5CX9CX12CX7C. Western blot analysis showed that the abundance of HoCBP76 in the anterior, middle and posterior regions of the midgut was similar, indicating that HoCBP76 was secreted by the whole midgut epithelium, and confirmed the H. oblita PM belonged to the Type I PM. Immunolocalization analysis showed that HoCBP76 was mainly localized in the PM. The HoCBP76 is the first PM protein found in the H. oblita; however, its biochemical and physiological functions require further investigation. View Full-Text

Keywords: peritrophic membrane; Holotrichia oblita; cDNA expression library; chitin binding protein; HoCBP76 peritrophic membrane; Holotrichia oblita; cDNA expression library; chitin binding protein; HoCBP76





Author: Xiaomin Liu 1,3, Jie Li 4, Wei Guo 2,3,* , Ruijun Li 3, Dan Zhao 3 and Xinna Li 3

Source: http://mdpi.com/



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