Role of Aeromonas hydrophila Flagella Glycosylation in Adhesion to Hep-2 Cells, Biofilm Formation and Immune StimulationReport as inadecuate




Role of Aeromonas hydrophila Flagella Glycosylation in Adhesion to Hep-2 Cells, Biofilm Formation and Immune Stimulation - Download this document for free, or read online. Document in PDF available to download.

Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain





*

Author to whom correspondence should be addressed.



Abstract Polar flagellin proteins from Aeromonas hydrophila strain AH-3 serotype O34 were found to be O-glycosylated with a heterogeneous heptasaccharide glycan. Two mutants with altered light and strong polar flagella glycosylation still able to produce flagella were previously obtained, as well as mutants lacking the O34-antigen lipopolysaccharide LPS but with unaltered polar flagella glycosylation. We compared these mutants, altogether with the wild type strain, in different studies to conclude that polar flagella glycosylation is extremely important for A. hydrophila adhesion to Hep-2 cells and biofilm formation. Furthermore, the polar flagella glycosylation is an important factor for the immune stimulation of IL-8 production via toll receptor 5 TLR5. View Full-Text

Keywords: O-flagellin glycosylation; adhesion; biofilm; immune stimulation O-flagellin glycosylation; adhesion; biofilm; immune stimulation





Author: Susana Merino, Markus Wilhelms and Juan M. Tomás *

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents