Melatonin-Induced Temporal Up-Regulation of Gene Expression Related to Ubiquitin-Proteasome System UPS in the Human Malaria Parasite Plasmodium falciparumReport as inadecuate




Melatonin-Induced Temporal Up-Regulation of Gene Expression Related to Ubiquitin-Proteasome System UPS in the Human Malaria Parasite Plasmodium falciparum - Download this document for free, or read online. Document in PDF available to download.

1

Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São, São Paulo 05508-900, Brazil

2

Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, Brazil

3

Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32826, USA





*

Author to whom correspondence should be addressed.



Abstract There is an increasing understanding that melatonin and the ubiquitin- proteasome system UPS interact to regulate multiple cellular functions. Post-translational modifications such as ubiquitination are important modulators of signaling processes, cell cycle and many other cellular functions. Previously, we reported a melatonin-induced upregulation of gene expression related to ubiquitin-proteasome system UPS in Plasmodium falciparum, the human malaria parasite, and that P. falciparum protein kinase 7 influences this process. This implies a role of melatonin, an indolamine, in modulating intraerythrocytic development of the parasite. In this report we demonstrate by qPCR analysis, that melatonin induces gene upregulation in nine out of fourteen genes of the UPS, consisting of the same set of genes previously reported, between 4 to 5 h after melatonin treatment. We demonstrate that melatonin causes a temporally controlled gene expression of UPS members. View Full-Text

Keywords: melatonin; ubiquitin-proteasome-system; Plasmodium falciparum; malaria melatonin; ubiquitin-proteasome-system; Plasmodium falciparum; malaria





Author: Fernanda C. Koyama 1,2, Mauro F. Azevedo 2, Alexandre Budu 2, Debopam Chakrabarti 3 and Célia R. S. Garcia 2,*

Source: http://mdpi.com/



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