Detection of Serum Protein Biomarkers for the Diagnosis and Staging of HepatoblastomaReport as inadecuate




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1

Department of Pediatric Surgery, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China

2

Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou 450000, China

3

Institute of Cancer, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Jiefang Road, Hangzhou 310000, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Johannes Haybaeck

Abstract The present study aimed to identify serum biomarkers for the detection of hepatoblastoma HB. Serum samples were collected from 71 HB patients stage I, n = 19; stage II, n = 19, stage III, n = 19; and stage IV, n = 14 and 23 age- and sex-matched healthy children. Differential expression of serum protein markers were screened using surface-enhanced laser desorption-ionization time-of-flight mass spectrometry SELDI-TOF-MS, and the target proteins were isolated and purified using HPLC and identified using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry MALDI-TOF-MS, SEQUEST, and bioinformatics analysis. Differential protein expression was confirmed by enzyme-linked immunosorbent analysis ELISA. SELDI-TOF-MS screening identified a differentially expressed protein with an m-z of 9348 Da, which was subsequently identified as Apo A–I; its expression was significantly lower in the HB group as compared to the normal control group 1546.67 ± 757.81 vs. 3359.21 ± 999.36, respectively; p < 0.01. Although the expression level decreased with increasing disease stage, pair-wise comparison revealed significant differences in Apo A–I expression between the normal group and the HB subgroups p < 0.01. ELISA verified the reduced expression of Apo A–I in the HB group. Taken together, these results suggest that Apo A–I may represent a serum protein biomarker of HB. Further studies will assess the value of using Apo A–I expression for HB diagnosis and staging. View Full-Text

Keywords: hepatoblastom; SELDI-TOF-MS; MALDI-TOF-MS; 2D-LC-LTQ-MS; apolipoprotein A–I; protein biomarker hepatoblastom; SELDI-TOF-MS; MALDI-TOF-MS; 2D-LC-LTQ-MS; apolipoprotein A–I; protein biomarker





Author: Wei Zhao 1,†, Juan Li 2,†, Junjie Zhang 1, Pengfei Gao 1, Hang Pei 1, Lei Wang 1, Fei Guo 1, Jiekai Yu 3, Shu Zheng 3 and Jiaxiang Wang 1,*

Source: http://mdpi.com/



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