Calycosin Suppresses RANKL-Mediated Osteoclastogenesis through Inhibition of MAPKs and NF-κBReport as inadecuate




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1

Key Laboratory for Molecular and Chemical Genetics of Critical Human Diseases of Jilin Province, Jilin University Bethune Second Hospital, Changchun 130041, China

2

School of Life Sciences and Technology, Tongji University, Shanghai 200092, China

3

Key laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China

4

Department of Virus Disease Prevention and Control, Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Academic Editors: Gopinadhan Paliyath and Sanjay K. Srivastava

Abstract Calycosin, an isoflavonoid phytoestrogen, isolated from Radix Astragali, was reported to possess anti-tumor, anti-inflammation, and osteogenic properties, but its impact on osteoclast differentiation remains unclear. In this study, we examined the effects of calycosin on osteoclastogenesis induced by RANKL. The results showed that calycosin significantly inhibited RANKL-induced osteoclast formation from primary bone marrow macrophages BMMs. Calycosin also dose-dependently suppressed the formation of bone resorption pits by mature osteoclasts. In addition, the expression of osteoclatogenesis-related genes, including cathepsin K CtsK, tartrate-resistant acid phosphatase TRAP, and MMP-9, was significantly inhibited by calycosin. Furthermore, the results indicated that calycosin down-regulated the expression levels of NFATc1 and c-Fos through suppressing the activation of NF-κB and MAPKs. Our results indicate that calycosin has an inhibitory role in the bone loss by preventing osteoclast formation, as well as its bone resorptive activity. Therefore, calycosin may be useful as a therapeutic reagent for bone loss-associated diseases. View Full-Text

Keywords: calycosin; osteoclast; bone resorption; NFATc1; c-Fos; NF-κB; MAPKs calycosin; osteoclast; bone resorption; NFATc1; c-Fos; NF-κB; MAPKs





Author: Gui-Hua Quan 1,†, Hongbing Wang 2,†, Jinjin Cao 3, Yuxin Zhang 3, Donglin Wu 4, Qisheng Peng 3, Ning Liu 1,* and Wan-Chun Sun 3,*

Source: http://mdpi.com/



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