Ras-Related Nuclear Protein Ran3B Gene Is Involved in Hormone Responses in the Embryogenic Callus of Dimocarpus longan Lour.Report as inadecuate




Ras-Related Nuclear Protein Ran3B Gene Is Involved in Hormone Responses in the Embryogenic Callus of Dimocarpus longan Lour. - Download this document for free, or read online. Document in PDF available to download.

Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China





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Academic Editor: Jianhua Zhu

Abstract Ras-related guanosine triphosphate GTP-binding nuclear protein Ran GTPases function as molecular switches and regulate diverse cellular events in eukaryotes. Our previous work suggested that DlRan3B is active during longan Dimocarpus longan Lour. somatic embryogenesis SE processes. Herein, subcellular localization of DlRan3B was found to be localized in the nucleus and expression profiling of DlRan3B was performed during longan SE and after exposure to plant hormones indoleacetic acid IAA, gibberellin A3 GA3, salicylic acid SA, methyl jasmonte MeJA, and abscisic acid ABA. We cloned and sequenced 1569 bp of 5′-flanking sequence of DlRan3B GenBank: JQ279697. Bioinformatic analysis indicated that the promoter contained plant hormone-related regulatory elements. Deletion analysis and responses to hormones identified stimulative and repressive regulatory elements in the DlRan3B promoter. The key elements included those responding to auxin, gibberellin, SA, MeJA, and ABA. DlRan3B was located in the nucleus and accumulated in the late stage of longan SE. The expression of DlRan3B was significantly induced by IAA, GA3, and ABA, but suppressed by SA and MeJA. Promoter transcription was induced by IAA and GA3, but suppressed by SA. Thus, DlRan3B might participate in auxin, gibberellin, and ABA responses during longan late SE, and DlRan3B is involved in phytohormone responsiveness. View Full-Text

Keywords: Ran GTPases; DlRan3B; SE; gene expression; promoter deletion analysis Ran GTPases; DlRan3B; SE; gene expression; promoter deletion analysis





Author: Qilin Tian, Yuling Lin, Dongmin Zhang, Ruilian Lai and Zhongxiong Lai *

Source: http://mdpi.com/



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