Visualization of Alternative Functional Configurations of Influenza Virus Hemagglutinin Facilitates Rapid Selection of Complementing Vaccines in Emergency SituationsReport as inadecuate


Visualization of Alternative Functional Configurations of Influenza Virus Hemagglutinin Facilitates Rapid Selection of Complementing Vaccines in Emergency Situations


Visualization of Alternative Functional Configurations of Influenza Virus Hemagglutinin Facilitates Rapid Selection of Complementing Vaccines in Emergency Situations - Download this document for free, or read online. Document in PDF available to download.

1

Veterinary Virologist and Consultant, Cairo 11441, Egypt

2

Virology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt





*

Author to whom correspondence should be addressed.



Academic Editors: Hsin-Fu Liu and Jih-Hui Lin

Abstract Successful immunization against avian influenza virus AIV requires eliciting an adequate polyclonal response to AIV hemagglutinin HA subunit 1 HA1 epitopes. Outbreaks of highly-pathogenic HP AIV subtype H5N1 can occur in vaccinated flocks in many endemic areas. Protection against emerging AIV is partly hindered by the limitations of vaccine production and transport, the use of leaky vaccines, and the use of multiple, and often antigenically-diverse, vaccines. It was hypothesized that the majority of alternative functional configurations AFC within the AIV HA1 can be represented by the pool of vaccine seed viruses currently in production because only a finite number of AFC are possible within each substructure of the molecule. Therefore, combinations of commercial vaccines containing complementing structural units CSU to each HA1 substructure can elicit responses to the totality of a given emerging AIV HA1 substructure isoforms. Analysis of homology-based 3D models of vaccine seed and emerging viruses facilitated the definition of HA1 AFC isoforms. CSU-based plots were used to predict which commercial vaccine combinations could have been used to cover nine selected AFC isoforms on recent Egyptian HP AIV H5N1 outbreak viruses. It is projected that expansion of the vaccine HA1 3D model database will improve international emergency responses to AIV. View Full-Text

Keywords: 3D modeling; alternative functional configurations; anchor sites; avian influenza virus; complementing structural units; escape mutants; fitness condition; hemagglutinin; vaccine combinations; vulnerable sites 3D modeling; alternative functional configurations; anchor sites; avian influenza virus; complementing structural units; escape mutants; fitness condition; hemagglutinin; vaccine combinations; vulnerable sites





Author: Ashraf Metwally 1 and Ausama Yousif 2,*

Source: http://mdpi.com/



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