Synaptonemal Complex Length Variation in Wild-Type Male MiceReport as inadecuate




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1

Johns Hopkins University, Baltimore, MD 21218, USA

2

Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract Meiosis yields haploid gametes following two successive divisions of a germ cell in the absence of intervening DNA replication. Balanced segregation of homologous chromosomes in Meiosis I is aided by a proteinaceous structure, the synaptonemal complex SC. The objective of this study was to determine total average autosomal SC lengths in spermatocytes in three commonly used mouse strains 129S4-SvJae, C57BL-6J, and BALB-c. Our experiments revealed that the total autosomal SC length in BALB-c spermatocytes is 9% shorter than in the two other strains. Shorter SCs are also observed in spermatocytes of BALB-c × 129S4-SvJae and C57BL-6J × BALB-c F1 hybrids suggesting a genetic basis of SC length regulation. Along these lines, we studied expression of a selected group of genes implicated in meiotic chromosome architecture. We found that BALB-c testes express up to 6-fold less of Rec8 mRNA and 4-fold less of REC8 protein. These results suggest that the mechanism that defines the SC length operates via a REC8‑dependent process. Finally, our results demonstrate that genetic background can have an effect on meiotic studies in mice. View Full-Text

Keywords: synaptonemal complex; meiosis; mouse; genetic background; synapsis; REC8 synaptonemal complex; meiosis; mouse; genetic background; synapsis; REC8





Author: Neil M. Vranis 1,†, Godfried W. Van der Heijden 2,†, Safia Malki 2 and Alex Bortvin 2,*

Source: http://mdpi.com/



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