High Content Imaging HCI on Miniaturized Three-Dimensional 3D Cell CulturesReport as inadecuate




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Department of Chemical and Biomedical Engineering, Cleveland State University, 1960 East 24th Street Cleveland, Ohio, OH 44115-2214, USA





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Academic Editor: Christophe A. Marquette

Abstract High content imaging HCI is a multiplexed cell staining assay developed for better understanding of complex biological functions and mechanisms of drug action, and it has become an important tool for toxicity and efficacy screening of drug candidates. Conventional HCI assays have been carried out on two-dimensional 2D cell monolayer cultures, which in turn limit predictability of drug toxicity-efficacy in vivo; thus, there has been an urgent need to perform HCI assays on three-dimensional 3D cell cultures. Although 3D cell cultures better mimic in vivo microenvironments of human tissues and provide an in-depth understanding of the morphological and functional features of tissues, they are also limited by having relatively low throughput and thus are not amenable to high-throughput screening HTS. One attempt of making 3D cell culture amenable for HTS is to utilize miniaturized cell culture platforms. This review aims to highlight miniaturized 3D cell culture platforms compatible with current HCI technology. View Full-Text

Keywords: high content imaging; three-dimensional 3D cell culture; miniaturized cell-based assay; predictive toxicology high content imaging; three-dimensional 3D cell culture; miniaturized cell-based assay; predictive toxicology





Author: Pranav Joshi and Moo-Yeal Lee *

Source: http://mdpi.com/



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