Cancer-Cells on Chip for Label-Free Detection of Secreted MoleculesReport as inadecuate

Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules - Download this document for free, or read online. Document in PDF available to download.

Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires—Institut de Chimie et Biochimie Moléculaires et Supramoléculaires—Université Claude Bernard Lyon 1, Villeurbanne 69100, France


Author to whom correspondence should be addressed.

Academic Editor: Roberto Pilloton

Abstract In the present report, we are making the proof of concept of cell small populations from 1 to 100 cells spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume 30 nL, directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging SPRi experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen PSA and β-2-microglobulin B2M secreted by prostate cancer cells following induction by dihydrotestosterone DHT. Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip. View Full-Text

Keywords: biosensor; cell microarray; cell encapsulation; SPRi; real-time biosensor; cell microarray; cell encapsulation; SPRi; real-time

Author: Ophélie I. Berthuy, Loïc J. Blum and Christophe A. Marquette *



Related documents