Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance SPR Technique: A Novel Method to Assess Biomolecular InteractionsReport as inadecuate




Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance SPR Technique: A Novel Method to Assess Biomolecular Interactions - Download this document for free, or read online. Document in PDF available to download.

Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, USA





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Academic Editors: Nicole Jaffrezic-Renault and Carole Chaix

Abstract Flavonoids are common polyphenolic compounds widely distributed in fruits and vegetables. These pigments have important pharmacological relevance because emerging research suggests possible anti-cancer and anti-inflammatory properties as well other beneficial health effects. These compounds are relatively hydrophobic molecules, suggesting the role of blood transport proteins in their delivery to tissues. In this study, we assess the binding interactions of four flavonoids kaempferol, luteolin, quercetin, and resveratrol with human serum albumin HSA, the most abundant protein in the blood, and with glutathione S-transferase pi isoform-1 GSTP1, an enzyme with well-characterized hydrophobic binding sites that plays an important role in detoxification of xenobiotics with reduced glutathione, using a novel Taylor dispersion surface plasmon resonance SPR technique. For the first time, HSA sites revealed a high-affinity binding site for flavonoid interactions. Out of the four flavonoids that we examined, quercetin and kaempferol showed the strongest equilibrium binding affinities KD of 63 ± 0.03 nM and 37 ± 0.07 nM, respectively. GSTP1 displayed lower affinities in the micromolar range towards all of the flavonoids tested. The interactions of flavonoids with HSA and GSTP1 were studied successfully using this novel SPR assay method. The new method is compatible with both kinetic and equilibrium analyses. View Full-Text

Keywords: flavonoids; age-related macular degeneration; nutraceutical; taylor dispersion; human serum albumin; glutathione s-transferase pi isoform-1 flavonoids; age-related macular degeneration; nutraceutical; taylor dispersion; human serum albumin; glutathione s-transferase pi isoform-1





Author: Preejith P. Vachali, Binxing Li, Brian M. Besch and Paul S. Bernstein *

Source: http://mdpi.com/



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