Glutamine-Glutamate Cycle Flux Is Similar in Cultured Astrocytes and Brain and Both Glutamate Production and Oxidation Are Mainly Catalyzed by Aspartate AminotransferaseReport as inadecuate


Glutamine-Glutamate Cycle Flux Is Similar in Cultured Astrocytes and Brain and Both Glutamate Production and Oxidation Are Mainly Catalyzed by Aspartate Aminotransferase


Glutamine-Glutamate Cycle Flux Is Similar in Cultured Astrocytes and Brain and Both Glutamate Production and Oxidation Are Mainly Catalyzed by Aspartate Aminotransferase - Download this document for free, or read online. Document in PDF available to download.

1

Laboratory of Brain Metabolic Diseases, Institute of Metabolic Disease Research and Drug Development, China Medical University, Liaoning 110000, China

2

Magnetic Resonance Research Center, Radiology and Biomedical Engineering, Yale University, New Haven, CT 06520, USA



Private address: RR2, Box 245, Gilmour, ON K0L 1W0, Canada.





*

Author to whom correspondence should be addressed.



Academic Editors: Arthur J.L. Cooper and Thomas M. Jeitner

Abstract The glutamine-glutamate cycle provides neurons with astrocyte-generated glutamate-γ-aminobutyric acid GABA and oxidizes glutamate in astrocytes, and it returns released transmitter glutamate-GABA to neurons after astrocytic uptake. This review deals primarily with the glutamate-GABA generation-oxidation, although it also shows similarity between metabolic rates in cultured astrocytes and intact brain. A key point is identification of the enzymes converting astrocytic α-ketoglutarate to glutamate and vice versa. Most experiments in cultured astrocytes, including those by one of us, suggest that glutamate formation is catalyzed by aspartate aminotransferase AAT and its degradation by glutamate dehydrogenase GDH. Strongly supported by results shown in Table 1 we now propose that both reactions are primarily catalyzed by AAT. This is possible because the formation occurs in the cytosol and the degradation in mitochondria and they are temporally separate. High glutamate-glutamine concentrations abolish the need for glutamate production from α-ketoglutarate and due to metabolic coupling between glutamate synthesis and oxidation these high concentrations render AAT-mediated glutamate oxidation impossible. This necessitates the use of GDH under these conditions, shown by insensitivity of the oxidation to the transamination inhibitor aminooxyacetic acid AOAA. Experiments using lower glutamate-glutamine concentration show inhibition of glutamate oxidation by AOAA, consistent with the coupled transamination reactions described here. View Full-Text

Keywords: aspartate aminotransferase; astrocyte culture; brain metabolism; glutamate-glutamine cycle; glutamate dehydrogenase; glutamate oxidation; glutamine synthetase; malate-aspartate shuttle; metabolic compartmentation; nitrogen balance aspartate aminotransferase; astrocyte culture; brain metabolism; glutamate-glutamine cycle; glutamate dehydrogenase; glutamate oxidation; glutamine synthetase; malate-aspartate shuttle; metabolic compartmentation; nitrogen balance





Author: Leif Hertz 1,† and Douglas L Rothman 2,*

Source: http://mdpi.com/



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