Label Free Detection of L-Glutamate Using Microfluidic Based Thermal BiosensorReport as inadecuate


Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor


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1

The Center for Biomedical Engineering and Rehabilitation Science, Louisiana Tech University, Ruston, LA 71272, USA

2

Department of Mechanical & Industrial Engineering, Qatar University, P.O. Box 2713, Doha, Qatar





*

Author to whom correspondence should be addressed.



Academic Editor: Anthony Guiseppi-Elie

Abstract A thermoelectric biosensor for the detection of L-glutamate concentration was developed. The thermoelectric sensor is integrated into a micro-calorimeter which measures the heat produced by biochemical reactions. The device contains a single flow channel that is 120 µm high and 10 mm wide with two fluid inlets and one fluid outlet. An antimony-bismuth Sb-Bi thermopile with high common mode rejection ratio is attached to the lower channel wall and measures the dynamic changes in the temperature when L-glutamate undergoes oxidative deamination in the presence of glutamate oxidase GLOD. The thermopile has a Seebeck coefficient of ~7 µV·m·K−1. The device geometry, together with hydrodynamic focusing, eliminates the need of extensive temperature control. Layer-by-layer assembly is used to immobilize GLOD on the surface of glass coverslips by alternate electrostatic adsorption of polyelectrolyte and GLOD. The impulse injection mode using a 6-port injection valve minimizes sample volume to 5 µL. The sensitivity of the sensor for glutamate is 17.9 nVs·mM−1 in the linear range of 0–54 mM with an R2 value of 0.9873. The lowest detection limit of the sensor for glutamate is 5.3 mM. View Full-Text

Keywords: thermoelectric; label-free; biosensor; L-glutamate; L-glutamate oxidase; layer-by-layer self-assembly thermoelectric; label-free; biosensor; L-glutamate; L-glutamate oxidase; layer-by-layer self-assembly





Author: Varun Lingaiah Kopparthy 1, Siva Mahesh Tangutooru 2 and Eric J. Guilbeau 1,*

Source: http://mdpi.com/



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