Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces SpeciesReport as inadecuate


Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species


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1

Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa

2

SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa





*

Author to whom correspondence should be addressed.



Academic Editor: Chih-Ching Huang

Abstract Cellulase and xylanase are in high demand for application in several industrial processes, consequently necessitating the bioprospecting and manipulation of microbes for novel and greater enzyme productivity. This study reports on the optimal conditions for cellulase and xylanase production by a marine bacterial isolate from Nahoon beach sediment, via the classical process of one variable per time. Furthermore, the inducing effect of mono- and polysaccharides on enzyme production was investigated. The 16S rDNA gene sequence analysis clearly assigned the isolate to the genus Streptomyces, and was deposited at the GenBank under the accession number KU171373. Cellulase and xylanase production was optimal at the following conditions: pH 6 and 8, incubation temperature of 40 and 35 °C, and agitation speed of 100 and 150 rpm, respectively. Under optimum conditions, 0.26 U-mL and 18.54 U-mL activities were attained at 60 and 48 h with specific productivity of 205 and 7417 U-g for cellulase and xylanase, respectively. Xylanase production was induced by the entire mono- and polysaccharides tested, while cellulase production was induced by some. The results from this study signify the resourcefulness of the Streptomyces strain for production of cellulase and xylanase of industrial importance. View Full-Text

Keywords: cellulase; xylanase; optimization; saccharides; Streptomyces; activity; production cellulase; xylanase; optimization; saccharides; Streptomyces; activity; production





Author: Evelyn N. Fatokun 1,2,* , Uchechukwu U. Nwodo 1,2 and Anthony I. Okoh 1,2

Source: http://mdpi.com/



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