Angiogenic and Osteogenic Coupling Effects of Deferoxamine-Loaded Polylactide-co-glycolide-Polyethylene glycol-Polylactide-co-glycolide NanoparticlesReport as inadecuate


Angiogenic and Osteogenic Coupling Effects of Deferoxamine-Loaded Polylactide-co-glycolide-Polyethylene glycol-Polylactide-co-glycolide Nanoparticles


Angiogenic and Osteogenic Coupling Effects of Deferoxamine-Loaded Polylactide-co-glycolide-Polyethylene glycol-Polylactide-co-glycolide Nanoparticles - Download this document for free, or read online. Document in PDF available to download.

1

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai 200233, China

2

Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Hidenori Otsuka

Abstract Angiogenesis and osteogenesis coupling processes are essential for bone regeneration, and human bone marrow stromal cells hBMSCs along with endothelial cells ECs are crucial participants. Deferoxamine DFO, a hypoxia-mimetic agent, could activate the hypoxia-inducible factor HIF-1α signaling pathway and trigger angiogenic and osteogenic effects in these cells. However, the lifetime of DFO is very short, thus a suitable delivery system is urgently needed. In this study, we encapsulated DFO in Polylactide-co-glycolide-Polyethylene glycol-Polylactide-co-glycolide PLGA-PEG-PLGA nanoparticles DFO-loaded NPs to realize its long-term angiogenic and osteogenic bioactivities. Surface morphology, size, size distribution of DFO-loaded NPs as well as DFO loading content LC, encapsulation efficiency EE and release profile were systematically evaluated. When hBMSCs were exposed to the vehicle with DFO concentration of 100 μM, cells showed good viability, increased HIF-1α expression and enhanced vascular endothelial growth factor VEGF secretion. The transcriptional levels of the angiogenic and osteogenic genes were also upregulated. Moreover, promoted alkaline phosphatase ALP activity further confirmed better osteogenic differentiation. Similarly, angiogenic activity of human umbilical vein endothelial cells HUVECs were enhanced after the addition of DFO-loaded NPs, evidenced by increased angiogenic genes expressions and tube formation. Taken together, DFO-loaded NPs could provide a sustained supply of DFO, with its angiogenic and osteogenic coupling effects preserved, which extends the potential of this system for bone defect repair. View Full-Text

Keywords: PLGA-PEG-PLGA; nanoparticles; deferoxamine; drug delivery system; angiogenesis; osteogenesis PLGA-PEG-PLGA; nanoparticles; deferoxamine; drug delivery system; angiogenesis; osteogenesis





Author: Manle Qiu 1,†, Chongyang Wang 1,†, Daoyun Chen 1,†, Chaoyong Shen 2, Huakun Zhao 1 and Yaohua He 1,*

Source: http://mdpi.com/



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