Characterization of Chemical Compounds with Antioxidant and Cytotoxic Activities in Bougainvillea x buttiana Holttum and Standl, var. Rose ExtractsReport as inadecuate


Characterization of Chemical Compounds with Antioxidant and Cytotoxic Activities in Bougainvillea x buttiana Holttum and Standl, var. Rose Extracts


Characterization of Chemical Compounds with Antioxidant and Cytotoxic Activities in Bougainvillea x buttiana Holttum and Standl, var. Rose Extracts - Download this document for free, or read online. Document in PDF available to download.

1

Facultad de Medicina, Universidad Autónoma del Estado de Morelos UAEM, Calle Leñeros, Esquina Iztaccíhuatl s-n, Cuernavaca, Morelos, C.P. 62350, Mexico

2

Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, C.P. 62250, Mexico





*

Author to whom correspondence should be addressed.



Academic Editor: Isabel C. F. R. Ferreira

Abstract Bougainvillea is widely used in traditional Mexican medicine to treat several diseases. This study was designed to characterize the chemical constituents of B. x buttiana extracts with antioxidant and cytotoxic activities using different solvents. The extraction solvents used were as follows: distilled water dH2O, methanol MeOH, acetone DMK, ethanol EtOH, ethyl acetate EtOAc, dichloromethane DCM, and hexane Hex 100% at an extraction temperature of 26 °C. Analysis of bioactive compounds present in the B. x buttiana extracts included the application of common phytochemical screening assays, GC-MS analysis, and cytotoxicity and antioxidant assays. The results show that the highest extraction yield was observed with water and methanol. The maximum total phenolic content amount and highest antioxidant potential were obtained when extraction with methanol was used. With the exceptions of water and ethanol extractions, all other extracts showed cytotoxicity ranging between 31% and 50%. The prevailing compounds in water, methanol, ethanol, and acetone solvents were as follows: 4H-pyran-4-one, 2,3-dihydro-3, 5-dihydroxy-6-methyl 2, 2-propenoic acid, 3-2-hydrophenyl-E- 3, and 3-O-methyl-d-glucose 6. By contrast, the major components in the experiments using solvents such as EtOH, DMK, EtOAc, DCM, and Hex were n-hexadecanoic acid 8, 9,12-octadecadienoic acid Z,Z 12; 9-octadecenoic acid E- 13, and stigmasta-5,22-dien-3-ol 28. View Full-Text

Keywords: solvent extraction; total phenolic content TPC; antioxidant; cytotoxic; Bougainvillea x buttiana solvent extraction; total phenolic content TPC; antioxidant; cytotoxic; Bougainvillea x buttiana





Author: Rodolfo Abarca-Vargas 1, Carlos F. Peña Malacara 2 and Vera L. Petricevich 1,*

Source: http://mdpi.com/



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