Hydroxybenzoic Acids Are Significant Contributors to the Antioxidant Effect of Borututu Bark, Cochlospermum angolensis Welw. ex OlivReport as inadecuate


Hydroxybenzoic Acids Are Significant Contributors to the Antioxidant Effect of Borututu Bark, Cochlospermum angolensis Welw. ex Oliv


Hydroxybenzoic Acids Are Significant Contributors to the Antioxidant Effect of Borututu Bark, Cochlospermum angolensis Welw. ex Oliv - Download this document for free, or read online. Document in PDF available to download.

College of Pharmacy, Chicago State University, Chicago, IL 60628, USA





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Academic Editors: Isabel C. F. R. Ferreira and Stanley Omaye

Abstract Borututu Cochlospermum angolensis is an African tree whose bark has recently emerged as a herbal dietary supplement with claims for antioxidant activity. In order to substantiate the claimed activity of borututu supplements, we performed an activity-guided fractionation of the total extract utilizing a 1,1-diphenyl-2-picrylhydrazyl DPPH free radical scavenging assay. Subsequent flash and centrifugal chromatography resulted in the isolation of gallic acid 1 and protocatechuic acid 2 as the main antioxidant constituents. Two apocarotenoids and one flavonoid were also isolated from the chloroform fraction and were identified as cochloxanthin 3, dihydrocochloxanthin 4, and 7,4′-dimethyltaxifolin 5, respectively. A High-performance liquid chromatography HPLC method was also developed for fingerprinting borututu samples, with Compounds 1–4 suggested as chemical markers for quality control purposes. View Full-Text

Keywords: flash chromatography; centrifugal thin-layer chromatography; phenolic acids; apocarotenoids; antioxidants; DPPH scavenging; HPLC fingerprinting flash chromatography; centrifugal thin-layer chromatography; phenolic acids; apocarotenoids; antioxidants; DPPH scavenging; HPLC fingerprinting





Author: Ehab A. Abourashed * and Hao Wen Fu

Source: http://mdpi.com/



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