Vol 9: Zebrafish 3-O-Sulfotransferase-4 Generated Heparan Sulfate Mediates HSV-1 Entry and Spread.Report as inadecuate



 Vol 9: Zebrafish 3-O-Sulfotransferase-4 Generated Heparan Sulfate Mediates HSV-1 Entry and Spread.


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This article is from PLoS ONE, volume 9.AbstractRare modification of heparan sulfate HS by glucosaminyl 3-O sulfotransferase 3-OST isforms generates an entry receptor for herpes simplex virus type-1 HSV-1. In the zebrafish ZF model multiple 3-OST isoforms are differentially expressed. One such isoform is 3-OST-4 which is widely expressed in the central nervous system of ZF. In this report we characterize the role of ZF encoded 3-OST-4 isoform for HSV-1 entry. Expression of ZF 3-OST-4 into resistant Chinese hamster ovary CHO-K1 cells promoted susceptibility to HSV-1 infection. This entry was 3-O sulfated HS 3-OS HS dependent as pre-treatment of ZF 3-OST-4 cells with enzyme HS lyases heparinase II-III significantly reduced HSV-1 entry. Interestingly, co-expression of ZF 3-OST-4 along with ZF 3-OST-2 which is also expressed in brain rendered cells more susceptible to HSV-1 than 3-OST-4 alone. The role of ZF-3-OST-4 in the spread of HSV-1 was also evaluated as CHO-K1 cells that expressed HSV-1 glycoproteins fused with ZF 3-OST-4 expressing effector CHO-K1 cells. Finally, adding further evidence ZF 3-OST-4 mediated HSV-1 entry was inhibited by anti-3O HS G2 peptide. Taken together our results demonstrate a role for ZF 3-OST-4 in HSV-1 pathogenesis and support the use of ZF as a model to study it.



Author: Antoine, Thessicar E.; Yakoub, Abraam; Maus, Erika; Shukla, Deepak; Tiwari, Vaibhav

Source: https://archive.org/







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