Vol 9: ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes.Report as inadecuate



 Vol 9: ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes.


Vol 9: ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes. - Download this document for free, or read online. Document in PDF available to download.

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This article is from Nanoscale Research Letters, volume 9.AbstractIt has been documented in in vitro studies that zinc oxide nanoparticles ZnO NPs are capable of inducing oxidative stress, which plays a crucial role in ZnO NP-mediated apoptosis. However, the underlying molecular mechanism of apoptosis in neurocytes induced by ZnO NP exposure was not fully elucidated. In this study, we investigated the potential mechanisms of apoptosis provoked by ZnO NPs in cultured primary astrocytes by exploring the molecular signaling pathways triggered after ZnO NP exposure. ZnO NP exposure was found to reduce cell viability in MTT assays, increase lactate dehydrogenase LDH release, stimulate intracellular reactive oxygen species ROS generation, and elicit caspase-3 activation in a dose- and time-dependent manner. Apoptosis occurred after ZnO NP exposure as evidenced by nuclear condensation and polyADP-ribose polymerase-1 PARP cleavage. A decrease in mitochondrial membrane potential MMP with a concomitant increase in the expression of Bax-Bcl-2 ratio suggested that the mitochondria also mediated the pathway involved in ZnO NP-induced apoptosis. In addition, exposure of the cultured cells to ZnO NPs led to phosphorylation of c-Jun N-terminal kinase JNK, extracellular signal-related kinase ERK, and p38 mitogen-activated protein kinase p38 MAPK. Moreover, JNK inhibitor SP600125 significantly reduced ZnO NP-induced cleaved PARP and cleaved caspase-3 expression, but not ERK inhibitor U0126 or p38 MAPK inhibitor SB203580, indicating that JNK signaling pathway is involved in ZnO NP-induced apoptosis in primary astrocytes.



Author: Wang, Jieting; Deng, Xiaobei; Zhang, Fang; Chen, Deliang; Ding, Wenjun

Source: https://archive.org/



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