Vol 29: ViralFusionSeq: accurately discover viral integration events and reconstruct fusion transcripts at single-base resolution.Report as inadecuate



 Vol 29: ViralFusionSeq: accurately discover viral integration events and reconstruct fusion transcripts at single-base resolution.


Vol 29: ViralFusionSeq: accurately discover viral integration events and reconstruct fusion transcripts at single-base resolution. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 29: ViralFusionSeq: accurately discover viral integration events and reconstruct fusion transcripts at single-base resolution.
This article is from Bioinformatics, volume 29.AbstractSummary: Insertional mutagenesis from virus infection is an important pathogenic risk for the development of cancer. Despite the advent of high-throughput sequencing, discovery of viral integration sites and expressed viral fusion events are still limited. Here, we present ViralFusionSeq VFS, which combines soft-clipping information, read-pair analysis and targeted de novo assembly to discover and annotate viral–human fusions. VFS was used in an RNA-Seq experiment, simulated DNA-Seq experiment and re-analysis of published DNA-Seq datasets. Our experiments demonstrated that VFS is both sensitive and highly accurate.Availability: VFS is distributed under GPL version 3 at http:-hkbic.cuhk.edu.hk-software-viralfusionseqContact: tf.chan@cuhk.edu.hkSupplementary information: Supplementary data are available at Bioinformatics Online



Author: Li, Jing-Woei; Wan, Raymond; Yu, Chi-Shing; Co, Ngai Na; Wong, Nathalie; Chan, Ting-Fung

Source: https://archive.org/







Related documents