Vol 8: Upregulation of inflammatory genes in the nasal mucosa of patients undergoing endonasal dacryocystorhinostomy.Report as inadecuate



 Vol 8: Upregulation of inflammatory genes in the nasal mucosa of patients undergoing endonasal dacryocystorhinostomy.


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This article is from Clinical Ophthalmology Auckland, N.Z., volume 8.AbstractBackground: Epiphora is a common complaint of nasolacrimal duct obstruction NLDO in adults. The precise pathogenesis of NLDO is still unknown, but inflammatory processes are believed to be predisposing factors. Endoscopic dacryocystorhinostomy EN-DCR is an effective surgical technique for treating symptomatic NLDO. The purpose of the procedure is to relieve the patient’s symptoms by creating an opening, ie, a rhinostoma, between the lacrimal sac and the nasal cavity. Although the success rates after EN-DCR are high, the procedure sometimes fails due to onset of a fibrotic process at the rhinostomy site. The aim of this prospective comparative study was to investigate inflammation-related gene expression in the nasal mucosa at the rhinostomy site. Methods: Ten participants were consecutively recruited from eligible adult patients who underwent primary powered EN-DCR five patients or septoplasty five controls. Nasal mucosa specimens were taken from the rhinostomy site at the beginning of surgery for analysis of gene expression. Specimens were taken from the same site on the lateral nasal wall for controls. Quantitative reverse transcription polymerase chain reaction qRT-PCR was performed for the inflammatory genes interleukin IL-6, IL-1β, and CCL2, and because of a clear trend of increased inflammation in the EN-DCR samples, a wider PCR array was performed to compare inflammation-related gene expression in EN-DCR subjects and corresponding controls. Results: Our qRT-PCR results revealed a clear trend of increased transcription of IL-6, IL-1β, and CCL2 P=0.03. The same trend was also evident in the PCR array, which additionally revealed notable differences between EN-DCR subjects and controls with regard to expression of several other inflammation-related mediators. At 6-month follow-up, the success rate after primary EN-DCR was 60%, ie, in three of five patients. Conclusion: The present study demonstrates that there is an intense inflammation gene expression response in the nasal mucosa of patients undergoing EN-DCR.



Author: Penttila, Elina; Hyttinen, Juha MT; Hytti, Maria; Kauppinen, Anu; Smirnov, Grigori; Tuomilehto, Henri; Seppa, Juha; Nuutinen, Juhani; Kaarniranta, Kai

Source: https://archive.org/



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