Vol 9: Tcf3 Represses Wnt-Catenin Signaling and Maintains Neural Stem Cell Population during Neocortical Development.Report as inadecuate



 Vol 9: Tcf3 Represses Wnt-Catenin Signaling and Maintains Neural Stem Cell Population during Neocortical Development.


Vol 9: Tcf3 Represses Wnt-Catenin Signaling and Maintains Neural Stem Cell Population during Neocortical Development. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 9: Tcf3 Represses Wnt-Catenin Signaling and Maintains Neural Stem Cell Population during Neocortical Development.
This article is from PLoS ONE, volume 9.AbstractDuring mouse neocortical development, the Wnt–β-catenin signaling pathway plays essential roles in various phenomena including neuronal differentiation and proliferation of neural precursor cells NPCs. Production of the appropriate number of neurons without depletion of the NPC population requires precise regulation of the balance between differentiation and maintenance of NPCs. However, the mechanism that suppresses Wnt signaling to prevent premature neuronal differentiation of NPCs is poorly understood. We now show that the HMG box transcription factor Tcf3 also known as Tcf7l1 contributes to this mechanism. Tcf3 is highly expressed in undifferentiated NPCs in the mouse neocortex, and its expression is reduced in intermediate neuronal progenitors INPs committed to the neuronal fate. We found Tcf3 to be a repressor of Wnt signaling in neocortical NPCs in a reporter gene assay. Tcf3 bound to the promoter of the proneural bHLH gene Neurogenin1 Neurog1 and repressed its expression. Consistent with this, Tcf3 repressed neuronal differentiation and increased the self-renewal activity of NPCs. We also found that Wnt signal stimulation reduces the level of Tcf3, and increases those of Tcf1 also known as Tcf7 and Lef1, positive mediators of Wnt signaling, in NPCs. Together, these results suggest that Tcf3 antagonizes Wnt signaling in NPCs, thereby maintaining their undifferentiated state in the neocortex and that Wnt signaling promotes the transition from Tcf3-mediated repression to Tcf1-Lef1-mediated enhancement of Wnt signaling, constituting a positive feedback loop that facilitates neuronal differentiation.



Author: Kuwahara, Atsushi; Sakai, Hiroshi; Xu, Yuanjiang; Itoh, Yasuhiro; Hirabayashi, Yusuke; Gotoh, Yukiko

Source: https://archive.org/



DOWNLOAD PDF




Related documents