Vol 9: Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study.Report as inadecuate



 Vol 9: Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study.


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This article is from PLoS ONE, volume 9.AbstractBackground: We derived mesenchymal stem cells MSCs from rat induced pluripotent stem cells iPSCs and transduced them with tumor necrosis factor alpha-stimulated gene-6 TSG-6, to test whether TSG-6 overexpression would boost the therapeutic effects of iPSC-derived MSCs in experimental periodontitis. Methods: A total of 30 female Sprague-Dawley SD rats were randomly divided into four groups: healthy control group Group-N, n = 5, untreated periodontitis group Group-P, n = 5, iPS-MSCs-treated and iPSC-MSCs-TSG-6-treated periodontitis groups Group-P1 and P2, n = 10 per group. Experimental periodontitis was established by ligature and infection with Porphyromonas gingivalis around the maxillae first molar bilaterally. MSC-like cells were generated from rat iPSCs, and transducted with TSG-6. iPSC-MSCs or iPSC-MSCs-TSG-6 were administrated to rats in Group-P1 or P2 intravenously and topically, once a week for three weeks. Blood samples were obtained one week post-injection for the analysis of serum pro-inflammatory cytokines. All animals were killed 3 months post-treatment; maxillae were then dissected for histological analysis, tartrate-resistant acid phosphatase TRAP staining, and morphological analysis of alveolar bone loss. Results: Administration of iPSC-MSC-TSG-6 significantly decreased serum levels of IL-1β and TNF-α in the Group-P2 rats 65.78 pg-ml and 0.56 pg-ml compared with those in Group-P 168.31 pg-ml and 1.15 pg-ml respectively p



Author: Yang, Heng; Aprecio, Raydolfo M.; Zhou, Xiaodong; Wang, Qi; Zhang, Wu; Ding, Yi; Li, Yiming

Source: https://archive.org/







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