Vol 15: Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration.Report as inadecuate



 Vol 15: Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration.


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This article is from BMC Musculoskeletal Disorders, volume 15.AbstractBackground: This study evaluated whether Schwann-like cells SLCs induced from bone marrow-derived mesenchymal stem cells BM-MSCs transplanted into acellular nerve grafts ANGs could repair nerve defects compared with nerve isografts and ANGs with BM-MSCs. Methods: BM-MSCs extracted, separated and purified from the bone marrow of rats, and some of the BM-MSCs were cultured with mixed induction agents that could induce BM-MSCs into SLCs. Either SLCs or BM-MSCs were seeded onto 10-mm ANGs, and the isografts were chosen as the control. The walking-track test, tibialis anterior muscle weight measurement, electrophysiological examination, toluidine blue staining, transmission electron micrographs and immunostaining of S-100 and VEGF in these three groups were evaluated in a 10-mm rat sciatic injury-repair model. Results: The walking-track test, tibialis anterior muscle weight measurement and electrophysiological examination of the sciatic nerve suggested the groups of ANGs with SLCs and isografts obtained better results than the BM-MSC group Pā€‰ā€‰0.05. All the histomorphometric analyses toluidine blue staining, transmission electron micrographs and immunostaining of S-100 and VEGF showed that there were more regenerating nerve fibers in the group of ANGs with SLCs than the BM-MSCs Pā€‰ā€‰0.05. Conclusions: SLCs seeded in ANGs and isografts show better functional regeneration compared with BM-MSCs seeded in ANGs. Additionally, SLCs combined with ANGs present almost the same outcome as the isografts. Therefore, SLCs with ANGs can be a good choice in nerve defect repairs.



Author: Fan, Lihong; Yu, Zefeng; Li, Jia; Dang, Xiaoqian; Wang, Kunzheng

Source: https://archive.org/



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