Vol 9: Restrictive Expression of Acid-Sensing Ion Channel 5 Asic5 in Unipolar Brush Cells of the Vestibulocerebellum.Report as inadecuate



 Vol 9: Restrictive Expression of Acid-Sensing Ion Channel 5 Asic5 in Unipolar Brush Cells of the Vestibulocerebellum.


Vol 9: Restrictive Expression of Acid-Sensing Ion Channel 5 Asic5 in Unipolar Brush Cells of the Vestibulocerebellum. - Download this document for free, or read online. Document in PDF available to download.

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This article is from PLoS ONE, volume 9.AbstractAcid-sensing ion channels Asic are ligand-gated ion channels in the Degenerin-Epithelial Na+ channel Deg-ENaC family. Asic proteins are richly expressed in mammalian neurons. Mammals express five Asic genes: Asic1-5. The gene product of Asic5 is an orphan member of the family about which little is known. To investigate Asic5 expression, we created an Asic5 reporter mouse. We find that Asic5 is chiefly expressed in the brain in the cerebellum, specifically in the ventral uvula and nodulus of the vestibulocerebellum. Here, Asic5 is restrictively expressed in a subset of interneurons in the granular layer. The locale, distinctive shape and immunohistochemical properties of these Asic5-expressing interneurons identify them as unipolar brush cells UBC. Asic5 is richly expressed in a subset of UBCs that also express the metabotropic glutamate receptor 1α mGluR1α but not those that express calretinin. Results from single cell RT-PCR and electrophysiological examination of these cells are consistent with this identity. Such observations are consistent with Asic5 playing a key role in the physiology of UBCs and in the function of the vestibulocerebellum.



Author: Boiko, Nina; Kucher, Volodymyr; Wang, Bin; Stockand, James D.

Source: https://archive.org/



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