Vol 7: Rapid purification of gram quantities of -sitosterol from a commercial phytosterol mixture.Report as inadecuate



 Vol 7: Rapid purification of gram quantities of -sitosterol from a commercial phytosterol mixture.


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This article is from BMC Research Notes, volume 7.AbstractBackground: β-Sitosterol, a plant sterol or phytosterol, has commercial uses in the nutraceutical and pharmaceutical industries, but is also employed frequently in biological research. Phytosterols always accumulate as mixtures, and obtaining highly pure β-sitosterol in larger quantities for biological assays has been a challenge. Findings: An improved method for the rapid purification of β-sitosterol from a commercial phytosterol extract is presented. Fractional crystallization of soybean oil yielded a soluble and an insoluble fraction. β-Sitosterol was purified by silica gel and Na-Y zeolite chromatography. Conclusion: The rapid and cost effective three-step purification described here afforded β-sitosterol in gram quantities with high purity 92% and yield 22%.



Author: Srividya, Narayanan; Heidorn, Deanna Brooke; Lange, Bernd Markus

Source: https://archive.org/



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