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Publisher: Optical Society of America

Issued date: 2013-11-04

Citation: Optics Express, 2013, 2122, 25912-25925.

ISSN: 1094-4087

DOI: 10.1364-OE.21.025912

Sponsor: This work waspartially supported by EC FP7 collaborative grant FMT-XCT and the Bill and Melinda Gatesfoundation. A.A. wishes to acknowledge support from Marie Curie IEF-2010-275137. J.R.wishes to acknowledge support from EC FP7 IMI project PREDICT-TB, and the EC FP7CIG grant HIGH-THROUGHPUT TOMO. D.D., S.Z. and J.T. acknowledge support from theNational Basic Research Program of China 973 Program under Grant 2011CB707700, theFellowship for Young International Scientists of the Chinese Academy of Sciences underGrant 2010Y2GA03, the National Natural Science Foundation of China under Grant81101084 and Instrument Developing Project of the Chinese Academy of Sciences underGrant No. YZ201164

Publisher version: http:-dx.doi.og-10.1364-OE.21.025912

Project: info:eu-repo-grantAgreement-EC-FP7-275137info:eu-repo-grantAgreement-EC-FP7-333632

Keywords: Fluorescence microscopy , Three-dimensional microscopy , Image reconstruction techniques , Light propagation in tissues , Tomography

Abstract:A new technique termed Helical Optical Projection Tomography hOPT has been developed with the aim to overcome some of the limitations of current 3D optical imaging techniques. hOPT is based on Optical Projection Tomography OPT with the major difference thaA new technique termed Helical Optical Projection Tomography hOPT has been developed with the aim to overcome some of the limitations of current 3D optical imaging techniques. hOPT is based on Optical Projection Tomography OPT with the major difference that there is a translation of the sample in the vertical direction during the image acquisition process, requiring a new approach to image reconstruction. Contrary to OPT, hOPT makes possible to obtain 3D-optical images of intact long samples without imposing limits on the sample length. This has been tested using hOPT to image long murine tissue samples such as spinal cords and large intestines. Moreover, 3D-reconstructed images of the colon of DSS-treated mice, a model for Inflammatory Bowel Disease, allowed the identification of the structural alterations. Finally, the geometry of the hOPT device facilitates the addition of a Selective Plane Illumination Microscopy SPIM arm, providing the possibility of delivering high resolution images of selected areas together with complete volumetric information+-





Author: Arranz, Alicia; Dong, Di; Zhu, Shouping; Rudin, Markus; Tsatsanis, Christos; Tian, Jie; Ripoll, Jorge

Source: http://e-archivo.uc3m.es


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Universidad Carlos III de Madrid Repositorio institucional e-Archivo http:--e-archivo.uc3m.es Área de Imagen e Instrumentación (BiiG) DBIAB - BIIG - Journal Articles 2013-11-04 Helical Optical Projection Tomography Arranz, Alicia Optical Society of America Optics Express, (2013), 21(22), 25912-25925. http:--hdl.handle.net-10016-17857 Descargado de e-Archivo, repositorio institucional de la Universidad Carlos III de Madrid © 2013 Optical Society of America This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA.
The paper can be found at the following URL on the OSA website: http:--dx.doi.org-10.1364-OE.21.025912.
Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law. Alicia Arranz, Di Dong, Shouping Zhu, Markus Rudin, Christos Tsatsanis, Jie Tian, and Jorge Ripoll, -Helical optical projection tomography,- Opt.
Express 21, 25912-25925 (2013) Helical optical projection tomography Alicia Arranz,1,8 Di Dong,2,8 Shouping Zhu,3 Markus Rudin,1 Christos Tsatsanis,4 Jie Tian2,3 and Jorge Ripoll5,6,7,* 1 Institute for Biomedical Engineering, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich, Switzerland 2 Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China 3 School of Life Sciences and Technology, Xidian University, Xian, Shaanxi 710071, China 4 Laboratory of Clinical Chemistry, School of Medicine, University of Crete, Heraklion 71003, Greece 5 Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (FORTH), 71110 Heraklion, Greece 6 Department of Bioengineering and Aerospace Engineering, Universidad Carlos III of Madrid, 28911 Madrid, Spain 7 Experimental Medicine and Surgery Unit, Instituto de Investigación Sanitaria del Hospital Gregorio Marañón, 28007 Madrid, Spain 8 These authors have contributed...





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