Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) Report as inadecuate




Effect of the combination of naa, kinetin and sucrose on the induction of somatic embryogenesis in lulo (solanum quitoense lam.) - Download this document for free, or read online. Document in PDF available to download.



Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA Plant Growth Regulators, PGRs, and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types hypocotyl and cotyledon were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 μM of NAA were added to the medium with sucrose 50.0 and 263.1 μM for the two explant types used. In lulo with spines, the highest percentage of embryonic structures 50% was observed with cotyledonary leaf explants and 50 μM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 μM of NAA 263.1 μM of sucrose 32%.

Tipo de documento: Artículo - Article

Información adicional: © Facultad de Agronomía, Universidad Nacional de Colombia Se autoriza la reproducción y citación del material que aparece en la revista, siempre y cuando se indique de manera explícita: nombre de la revista, nombre del autores, año, volumen, número y páginas del artículo fuente. Las ideas y afirmaciones consignadas por los autores están bajo su responsabilidad y no interpretan necesariamente las opiniones y políticas de la Universidad Nacional de Colombia. La mención de productos o firmas comerciales en la revista no implica una recomendación o apoyo por parte de la Universidad ni de la Facultad; el uso de tales productos debe ceñirse a las recomendaciones de las etiquetas.

Palabras clave: Embryo culture, recalcitrance, plant growth regulators, tissue culture, plant propagation, fruit.





Source: http://www.bdigital.unal.edu.co


Teaser



propagation and tissue culture Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo (Solanum quitoense Lam.) Efecto de la combinación de ANA, kinetina y sacarosa sobre la inducción de embriogénesis somática de lulo (Solanum quitoense Lam.) Hernando Criollo1, Margarita Perea2, Mariano Toribio3, and Johanna Muñoz4 ABSTRACT RESUMEN Lulo  is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis.
This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium.
Two lulo varieties, Solanum quitoense var.
septentrionale and S.  quitoense  var.  quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C.
The highest production percentage of the embryos was obtained when 50 µM of NAA were added to the medium with sucrose (50.0 and 263.1 µM) for the two explant types used.
In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 µM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 µM of NAA 263.1 µM of sucrose (32%). El lulo es una especie de gran importancia en la fruticultura colombiana, con grandes problemas fitosanitarios que requieren un agresivo programa de mejoramiento orientado a la producción de variedades tolerantes.
Estos programas necesitan establecer protocolos de propagación masiva de plantas de alta eficiencia como la embriogénesis somática.
Nuestras investigaciones se orientaron al estudio de l...






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