Vol 14: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity.Report as inadecuate



 Vol 14: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity.


Vol 14: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity. - Download this document for free, or read online. Document in PDF available to download.

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This article is from Genome Biology, volume 14.AbstractDevelopment of a highly reproducible and sensitive single-cell RNA sequencing RNA-seq method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase.



Author: Sasagawa, Yohei; Nikaido, Itoshi; Hayashi, Tetsutaro; Danno, Hiroki; Uno, Kenichiro D; Imai, Takeshi; Ueda, Hiroki R

Source: https://archive.org/



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