Effect of gamma rays on vegetative buds of physalis peruviana l Report as inadecuate




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In Colombia, studies carried out to establish modern methods for propagation and selection of plant of ribes gooseberry Physalis peruviana are scarce so, it is necessary to explore alternative techniques such as the application of ionizing radiation, which enables greater genetic variability and to obtain seedlings with phenotypic characteristics that could be beneficial to contribute to improve productivity. To induce chromosomal alterations, axillary buds of P. peruviana with 50, 100, :00 and M00 Gy of gamma radiation were irradiated and were cultured in MS medium supplemented with BA 0.1 mg•L-1 plus IBA 0.05 mg•L-1. T4e experiment was con- ducted using a complete random design, Vve tests each one witht thirty experimental units were carried out. After nine subcultures NY multiplication cycles, M0 days each, regenerated shoots of irradiated buds presented smaller viability than the regenerated non irradiated buds. The regenerated plantlets buds irradiated with 100 Gy performed  higher rooting percentage, shoot length and number of leaves , the lethal dose of M00 Gy inhibited the development of shoots and roots, althougt the explants remained viable. In cells taken from radical tips of regenerated plantlets of irradiated buds were observed bridges in anap4ase and telop4ase, laggards and isolated chromosomes, being higher the frequency of laggard chromosomes. The higher percentage of cells with chromosomal alterations was quantified in radical tips of regenerated plantlets of buds irradiated with :00  Gy. In greenhouse conditions, 86-YM.8Z of regenerated plantlets of irradiated or not buds were viable.Key words: cromosomal alterations, gamma radiation, in vitro culture, ionizing radiation, Physalis, plantlets., En Colombia los estudios para establecer métodos modernos de propagación y selección de materiales vegetativos de uchuva Physalis peruviana son escasos; por tanto, es necesario explorar técnicas alternativas como la aplicación de radiación ionizante, la cual permite ampliar la variabilidad genética, obtener plántulas con características fenotípicas favorables y contribuir al mejoramiento de la productividad. Con el objeto de inducir alteraciones cromosómicas, yemas axilares de P. peruviana cultivadas en medio MS suplementado con 0.1 mg-lt de BA y 0.05 mg-lt de AIB fueron irradiadas con 50, 100, 200 y 300 Grays Gy de radiación gamma. El experimento se llevó a cabo utilizando un diseo completamente al azar, con cinco tratamientos, cada uno con 30 unidades experimentales. Después de nueve subcultivos ciclos de multiplicación de 30 días cada uno, los microtallos regenerados de yemas irradiadas presentaron menor viabilidad que los regenerados de yemas no irradiadas. Las plántulas regeneradas de yemas irradiadas con 100 Gy presentaron mayor porcentaje de enraizamiento, longitud del tallo y número de hojas; la dosis de 300 Gy inhibió el desarrollo de tallos y primordios radicales, aunque los explantes permanecieron viables. En células de ápices radicales tomados de plántulas regeneradas de yemas irradiadas se observaron puentes en anafase y telofase, cromosomas rezagados y cromosomas aislados, siendo mayor la frecuencia de los cromosomas rezagados. El mayor porcentaje de células con alteraciones cromosómicas se cuantificó en ápices radicales de plántulas regeneradas de yemas irradiadas con 200 Gy. En invernadero, 86 93.8% de plántulas regeneradas de yemas irradiadas y no irradiadas fueron viables.

Tipo de documento: Artículo - Article

Palabras clave: Alteraciones cromosómicas, cultivo in vitro, plántulas, Physalis, radiación gamma, radiación ionizante, uchuva.





Source: http://www.bdigital.unal.edu.co


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Effect of gamma rays on vegetative buds of Physalis peruviana L. Efecto de rayos gamma sobre yemas vegetativas de Physalis peruviana L. Diana Patricia Caro-Melgarejo1†, Sandra Yaneth Estupián-Rincón1‡, Leidy Yanira RacheCardenal2††, and José Constantino Pacheco-Maldonado 3* 1Biologist, Research GroupBIOPLASMA-UPTC.
School of Biological Sciences.
Faculty of Basic Sciences.
Universidad Pedagógica y Tecnológica de Colombia.
Tunja, Boyacá, Colombia.
2Biologist, M.Sc., Professor Department of Biology and Microbiology, Universidad de Boyacá.
Tunja, Boyacá, Colombia.
3PhD in Biologyist, Senior School of Biological Sciences, Director Group BIOPLASMA-UPTC, Universidad Pedagógica y Tecnológica de Colombia.
Tunja, Boyacá, Colombia.
*Corresponding author: jocpach@gmail.com; †dianapcaro@yahoo.com; ‡sandraer22@hotmail.com ; ††leidyrache@yahoo.com Rec.: 08.08.11 Acept.: 15.12.12 Abstract In Colombia, studies carried out to establish modern methods for propagation and selection of cape gooseberry (Physalis peruviana) plant material are scarce, therefore it is necessary to explore alternative techniques such as the application of ionizing radiation, which enables greater genetic variability and to obtain seedlings with phenotypic characteristics that could be beneficial to contribute to improve productivity.
To induce chromosomal alterations, axillary buds of P.
peruviana with 50, 100, 200 and 300 Gy (Grays) of gamma radiation were irradiated and were cultured in MS medium supplemented with BA 0.1 mgL-1 plus IBA 0.05 mgL-1.
The experiment was conducted using a complete random design, five treatments each with thirty experimental units.
After nine subcultures (9 multiplication cycles, 30 days each), regenerated shoots from irradiated buds presented smaller viability than the ones regenerated from non-irradiated buds.
The regenerated plantlets from irradiated buds with 100 Gy had higher rooting percentage, shoot length and number of leaves; the dose of 3...






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